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Addgene inc 3xap1pgl3
3xap1pgl3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3xap1pgl3/3xAP1pGL3+(3xAP-1+in+pGL3-basic)+(Plasmid+%2340342)/pmc12446446-153-0-1
Average 93 stars, based on 65 article reviews
3xap1pgl3 - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Plasmid Preparation:

Article Title: PTEN depletion reduces H3K27me3 levels to promote epithelial-to-mesenchymal transition in epithelial colorectal cancer cells
Article Snippet: Images were acquired on a Leica SP8 scanning confocal system 470 with MDi8-inverted microscope with LASX software (Leica Microsystems). .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342 ; RRID:Addgene_40342) and has three canonical AP-1 binding sites driving luciferase gene expression [ ]. ..

Article Title: A microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 Activity
Article Snippet: Goat Anti-Rabbit (H+L)-HRP Conjugate (BioRad #170-6515, Hercules, CA, USA) and Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad #170-6516) were used as a secondary antibodies. .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342 ; RRID:Addgene_40342, accessed on 4 April 2023). ..

Article Title: JNK kinase regulates phosphorylation of HCoV-229E nucleocapsid protein.
Article Snippet: pLentiPGK Puro DEST JNK KTR AA Clover (Addgene plasmid #90238) and pLentiPGK Puro DEST JNK KTR Clover (Addgene plasmid # 59151) were a gift from Markus Covert. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono. pLVX-M-puro (Addgene plasmid #125839) was a gift from Boyi Gan. .. 3xAP1pGL3 (Addgene plasmid #40342) and pMIEG3-JunDN (Addgene plasmid #40350) were a gift from Alexander Dent. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259)were a gift from Didier Trono. pLVX-AP1-GFP and pLVX-JunDN-HA were cloned using Gibson assembly (New England Biolabs (NEB), E2611L) with inserts amplified with appropriate overhangs using Q5 high-fidelity DNA polymerase (NEB, M0491L). .. JNK and c-Jun inhibitors were obtained from MedChemExpress (MCE): JNK-IN-8 (HY-13319), Bentamapimod (HY-14761), SP600125 (HY-12041), AS601245 (HY-11010), T-5224 (HY-12270), SR 11302 (HY-15870), 1-Methyl-6-oxo-1,6-dihydropyridine-3-carboxylic acid (HY-N4226).

Article Title: Loss of DJ-1 function contributes to Parkinson's disease pathogenesis in mice via RACK1-mediated PKC activation and MAO-B upregulation.
Article Snippet: Parkinson’s disease (PD) is a common neurodegenerative motor disorder characterized by a dramatic reduction in pars compacta of substantia nigra dopaminergic neurons and striatal dopamine (DA) levels.. Mutations or deletions in the PARK7/DJ-1 gene are associated with an early-onset familial form of PD.. DJ-1 protein prevents neurodegeneration via its regulation of oxidative stress and mitochondrial function as well as its roles in transcription and signal transduction.

Article Title: JNK kinase regulates phosphorylation of HCoV-229E nucleocapsid protein
Article Snippet: pLentiPGK Puro DEST JNK KTR AA Clover (Addgene plasmid #90238) and pLentiPGK Puro DEST JNK KTR Clover (Addgene plasmid # 59151) were a gift from Markus Covert. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono. pLVX-M-puro (Addgene plasmid #125839) was a gift from Boyi Gan. .. 3xAP1pGL3 (Addgene plasmid #40342) and pMIEG3-JunDN (Addgene plasmid #40350) were a gift from Alexander Dent. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono. pLVX-AP1-GFP and pLVX-JunDN-HA were cloned using Gibson assembly (New England Biolabs (NEB), E2611L) with inserts amplified with appropriate overhangs using Q5 high-fidelity DNA polymerase (NEB, M0491L). .. JNK and c-Jun inhibitors were obtained from MedChemExpress (MCE): JNK-IN-8 (HY-13319), Bentamapimod (HY-14761), SP600125 (HY-12041), AS601245 (HY-11010), T-5224 (HY-12270), SR 11302 (HY-15870), 1-Methyl-6-oxo-1,6-dihydropyridine-3-carboxylic acid (HY-N4226).

Article Title: A microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 Activity.
Article Snippet: Goat Anti-Rabbit (H+L)-HRP Conjugate (BioRad #170-6515, Hercules, CA, USA) and Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad #170-6516) were used as a secondary antibodies. .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342; RRID:Addgene_40342, accessed on 4 April 2023). ..

Article Title: PTEN depletion reduces H3K27me3 levels to promote epithelial-to-mesenchymal transition in epithelial colorectal cancer cells.
Article Snippet: Images were acquired on a Leica SP8 scanning confocal system 470 with MDi8-inverted microscope with LASX software (Leica Microsystems). .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342; RRID:Addgene_40342) and has three canonical AP-1 binding sites driving luciferase gene expression [45]. ..

Binding Assay:

Article Title: PTEN depletion reduces H3K27me3 levels to promote epithelial-to-mesenchymal transition in epithelial colorectal cancer cells
Article Snippet: Images were acquired on a Leica SP8 scanning confocal system 470 with MDi8-inverted microscope with LASX software (Leica Microsystems). .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342 ; RRID:Addgene_40342) and has three canonical AP-1 binding sites driving luciferase gene expression [ ]. ..

Article Title: Loss of DJ-1 function contributes to Parkinson's disease pathogenesis in mice via RACK1-mediated PKC activation and MAO-B upregulation.
Article Snippet: Parkinson’s disease (PD) is a common neurodegenerative motor disorder characterized by a dramatic reduction in pars compacta of substantia nigra dopaminergic neurons and striatal dopamine (DA) levels.. Mutations or deletions in the PARK7/DJ-1 gene are associated with an early-onset familial form of PD.. DJ-1 protein prevents neurodegeneration via its regulation of oxidative stress and mitochondrial function as well as its roles in transcription and signal transduction.

Article Title: PTEN depletion reduces H3K27me3 levels to promote epithelial-to-mesenchymal transition in epithelial colorectal cancer cells.
Article Snippet: Images were acquired on a Leica SP8 scanning confocal system 470 with MDi8-inverted microscope with LASX software (Leica Microsystems). .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342; RRID:Addgene_40342) and has three canonical AP-1 binding sites driving luciferase gene expression [45]. ..

Luciferase:

Article Title: PTEN depletion reduces H3K27me3 levels to promote epithelial-to-mesenchymal transition in epithelial colorectal cancer cells
Article Snippet: Images were acquired on a Leica SP8 scanning confocal system 470 with MDi8-inverted microscope with LASX software (Leica Microsystems). .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342 ; RRID:Addgene_40342) and has three canonical AP-1 binding sites driving luciferase gene expression [ ]. ..

Article Title: Loss of DJ-1 function contributes to Parkinson's disease pathogenesis in mice via RACK1-mediated PKC activation and MAO-B upregulation.
Article Snippet: Parkinson’s disease (PD) is a common neurodegenerative motor disorder characterized by a dramatic reduction in pars compacta of substantia nigra dopaminergic neurons and striatal dopamine (DA) levels.. Mutations or deletions in the PARK7/DJ-1 gene are associated with an early-onset familial form of PD.. DJ-1 protein prevents neurodegeneration via its regulation of oxidative stress and mitochondrial function as well as its roles in transcription and signal transduction.

Article Title: PTEN depletion reduces H3K27me3 levels to promote epithelial-to-mesenchymal transition in epithelial colorectal cancer cells.
Article Snippet: Images were acquired on a Leica SP8 scanning confocal system 470 with MDi8-inverted microscope with LASX software (Leica Microsystems). .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342; RRID:Addgene_40342) and has three canonical AP-1 binding sites driving luciferase gene expression [45]. ..

Gene Expression:

Article Title: PTEN depletion reduces H3K27me3 levels to promote epithelial-to-mesenchymal transition in epithelial colorectal cancer cells
Article Snippet: Images were acquired on a Leica SP8 scanning confocal system 470 with MDi8-inverted microscope with LASX software (Leica Microsystems). .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342 ; RRID:Addgene_40342) and has three canonical AP-1 binding sites driving luciferase gene expression [ ]. ..

Article Title: PTEN depletion reduces H3K27me3 levels to promote epithelial-to-mesenchymal transition in epithelial colorectal cancer cells.
Article Snippet: Images were acquired on a Leica SP8 scanning confocal system 470 with MDi8-inverted microscope with LASX software (Leica Microsystems). .. 3xAP1pGL3 (3xAP-1 in pGL3-basic) was a gift from Alexander Dent (Addgene plasmid # 40342; http://n2t.net/addgene:40342; RRID:Addgene_40342) and has three canonical AP-1 binding sites driving luciferase gene expression [45]. ..

Clone Assay:

Article Title: JNK kinase regulates phosphorylation of HCoV-229E nucleocapsid protein.
Article Snippet: pLentiPGK Puro DEST JNK KTR AA Clover (Addgene plasmid #90238) and pLentiPGK Puro DEST JNK KTR Clover (Addgene plasmid # 59151) were a gift from Markus Covert. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono. pLVX-M-puro (Addgene plasmid #125839) was a gift from Boyi Gan. .. 3xAP1pGL3 (Addgene plasmid #40342) and pMIEG3-JunDN (Addgene plasmid #40350) were a gift from Alexander Dent. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259)were a gift from Didier Trono. pLVX-AP1-GFP and pLVX-JunDN-HA were cloned using Gibson assembly (New England Biolabs (NEB), E2611L) with inserts amplified with appropriate overhangs using Q5 high-fidelity DNA polymerase (NEB, M0491L). .. JNK and c-Jun inhibitors were obtained from MedChemExpress (MCE): JNK-IN-8 (HY-13319), Bentamapimod (HY-14761), SP600125 (HY-12041), AS601245 (HY-11010), T-5224 (HY-12270), SR 11302 (HY-15870), 1-Methyl-6-oxo-1,6-dihydropyridine-3-carboxylic acid (HY-N4226).

Article Title: JNK kinase regulates phosphorylation of HCoV-229E nucleocapsid protein
Article Snippet: pLentiPGK Puro DEST JNK KTR AA Clover (Addgene plasmid #90238) and pLentiPGK Puro DEST JNK KTR Clover (Addgene plasmid # 59151) were a gift from Markus Covert. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono. pLVX-M-puro (Addgene plasmid #125839) was a gift from Boyi Gan. .. 3xAP1pGL3 (Addgene plasmid #40342) and pMIEG3-JunDN (Addgene plasmid #40350) were a gift from Alexander Dent. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono. pLVX-AP1-GFP and pLVX-JunDN-HA were cloned using Gibson assembly (New England Biolabs (NEB), E2611L) with inserts amplified with appropriate overhangs using Q5 high-fidelity DNA polymerase (NEB, M0491L). .. JNK and c-Jun inhibitors were obtained from MedChemExpress (MCE): JNK-IN-8 (HY-13319), Bentamapimod (HY-14761), SP600125 (HY-12041), AS601245 (HY-11010), T-5224 (HY-12270), SR 11302 (HY-15870), 1-Methyl-6-oxo-1,6-dihydropyridine-3-carboxylic acid (HY-N4226).

Amplification:

Article Title: JNK kinase regulates phosphorylation of HCoV-229E nucleocapsid protein.
Article Snippet: pLentiPGK Puro DEST JNK KTR AA Clover (Addgene plasmid #90238) and pLentiPGK Puro DEST JNK KTR Clover (Addgene plasmid # 59151) were a gift from Markus Covert. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono. pLVX-M-puro (Addgene plasmid #125839) was a gift from Boyi Gan. .. 3xAP1pGL3 (Addgene plasmid #40342) and pMIEG3-JunDN (Addgene plasmid #40350) were a gift from Alexander Dent. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259)were a gift from Didier Trono. pLVX-AP1-GFP and pLVX-JunDN-HA were cloned using Gibson assembly (New England Biolabs (NEB), E2611L) with inserts amplified with appropriate overhangs using Q5 high-fidelity DNA polymerase (NEB, M0491L). .. JNK and c-Jun inhibitors were obtained from MedChemExpress (MCE): JNK-IN-8 (HY-13319), Bentamapimod (HY-14761), SP600125 (HY-12041), AS601245 (HY-11010), T-5224 (HY-12270), SR 11302 (HY-15870), 1-Methyl-6-oxo-1,6-dihydropyridine-3-carboxylic acid (HY-N4226).

Article Title: JNK kinase regulates phosphorylation of HCoV-229E nucleocapsid protein
Article Snippet: pLentiPGK Puro DEST JNK KTR AA Clover (Addgene plasmid #90238) and pLentiPGK Puro DEST JNK KTR Clover (Addgene plasmid # 59151) were a gift from Markus Covert. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono. pLVX-M-puro (Addgene plasmid #125839) was a gift from Boyi Gan. .. 3xAP1pGL3 (Addgene plasmid #40342) and pMIEG3-JunDN (Addgene plasmid #40350) were a gift from Alexander Dent. pCMVR8.74 (Addgene plasmid # 22036) and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono. pLVX-AP1-GFP and pLVX-JunDN-HA were cloned using Gibson assembly (New England Biolabs (NEB), E2611L) with inserts amplified with appropriate overhangs using Q5 high-fidelity DNA polymerase (NEB, M0491L). .. JNK and c-Jun inhibitors were obtained from MedChemExpress (MCE): JNK-IN-8 (HY-13319), Bentamapimod (HY-14761), SP600125 (HY-12041), AS601245 (HY-11010), T-5224 (HY-12270), SR 11302 (HY-15870), 1-Methyl-6-oxo-1,6-dihydropyridine-3-carboxylic acid (HY-N4226).

Generated:

Article Title: Loss of DJ-1 function contributes to Parkinson's disease pathogenesis in mice via RACK1-mediated PKC activation and MAO-B upregulation.
Article Snippet: Parkinson’s disease (PD) is a common neurodegenerative motor disorder characterized by a dramatic reduction in pars compacta of substantia nigra dopaminergic neurons and striatal dopamine (DA) levels.. Mutations or deletions in the PARK7/DJ-1 gene are associated with an early-onset familial form of PD.. DJ-1 protein prevents neurodegeneration via its regulation of oxidative stress and mitochondrial function as well as its roles in transcription and signal transduction.

Mutagenesis:

Article Title: Loss of DJ-1 function contributes to Parkinson's disease pathogenesis in mice via RACK1-mediated PKC activation and MAO-B upregulation.
Article Snippet: Parkinson’s disease (PD) is a common neurodegenerative motor disorder characterized by a dramatic reduction in pars compacta of substantia nigra dopaminergic neurons and striatal dopamine (DA) levels.. Mutations or deletions in the PARK7/DJ-1 gene are associated with an early-onset familial form of PD.. DJ-1 protein prevents neurodegeneration via its regulation of oxidative stress and mitochondrial function as well as its roles in transcription and signal transduction.



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